Increased cell product potency through improved storage solution 

Cell-based therapeutics have emerged as a critical aspect of regenerative medicine and transfusion. Cryopreservation is the process by which cell products are stored until use. Unfortunately, this is a challenging process for cells to survive and freezing protocols often remain suboptimal. Loss of cell viability after freezing reduces the success of therapies and can lead to poor outcomes for patients. New cryopreservation strategies and solutions are being developed that rely on either natural compounds or less toxic compounds to prevent the formation of ice and mitigate cell death. We hypothesize that the controlling the formation and growth of ice during cryopreservation using novel molecules will reduce cell toxicity and cryoinjuries and therefore improve post-thaw outcomes. In this work proposal, we will test new cryoprotectants with unique properties to control ice formation and ice crystals size. In Aim 1, the new cryoprotectants will be synthesized, their toxicity level on kidney and liver cells determined. Also, the capacity of these compounds to block ice crystal elongation will be measured. In Aim 2, we will investigate the capacity of these new cryoprotectants to protect blood stem cells grafts from freezing injuries using quality and functional assays. Altogether, this proposal offers complementary steps to improve the storage of cell products, which will benefit Canadian patients using cell products for life saving treatments.

PINEAULT, Nicolas

Canadian Blood Services

Intramural Research Grant Program

Ontario

$261,812